Insertion junction: LMJ.RY0402.231432_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231432
Chromosome:chromosome 15
Location:208478
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre15.g635350 PRM4,PRMT4,CARM1 (1 of 1) K05931 - histone-arginine methyltransferase CARM1 [EC:2.1.1.125] (CARM1, PRMT4); Protein-/Histone-arginine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGCACCCCAGCTGACGTTGGAGCAGGC
Internal bar code:GCCAGGACACGCGATTATACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:468
LEAP-Seq percent confirming:99.5623
LEAP-Seq n confirming:5687
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGGGGCTCAGAAAGAAGAG
Suggested primer 2:ATGATGATCCCTATGGCAGC