| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231436 |
| Chromosome: | chromosome 7 |
| Location: | 430403 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315250 | CSG5 | Chlamydomonas specific family G protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCGTTTAAACATGATCGTGAGCGTGATG |
| Internal bar code: | CATATCTACTGAGGCGAGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 595 |
| LEAP-Seq percent confirming: | 99.2272 |
| LEAP-Seq n confirming: | 19902 |
| LEAP-Seq n nonconfirming: | 155 |
| LEAP-Seq n unique pos: | 209 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGAGGAGAAGACCCTGAG |
| Suggested primer 2: | GAAGAGGAGGAAATGGAGGG |