| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231453 |
| Chromosome: | chromosome_12 |
| Location: | 216917 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g484900 | FAP83 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAAGTATACTTGATACGGACGATTGAAGAT |
| Internal bar code: | TTTATATCGCCCTGTGGCATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 98.825 |
| LEAP-Seq n confirming: | 3869 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCTCAACGATGAACGAGA |
| Suggested primer 2: | ATTGCCTCCAGACCTACCCT |