| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231468 |
| Chromosome: | chromosome 2 |
| Location: | 2845383 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g094700 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACAGATGACGCAGAGCCGCGACACCCA |
| Internal bar code: | AGAGTCGTTGTGGACTCGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 98.8785 |
| LEAP-Seq n confirming: | 7582 |
| LEAP-Seq n nonconfirming: | 86 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAATATACGCACAGCGA |
| Suggested primer 2: | ACTCCTGGTCGCACACCTAC |