| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231468 |
| Chromosome: | chromosome 5 |
| Location: | 2531303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235950 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTCCGCCGCTGCCGCCGGCGGCCCCTCT |
| Internal bar code: | CCGGGCGGCAGGCATTGTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 347 |
| LEAP-Seq percent confirming: | 99.7581 |
| LEAP-Seq n confirming: | 2474 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAAAAACCCACATCCTT |
| Suggested primer 2: | TGTCTGAGAGCCAGGTGATG |