| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231505 |
| Chromosome: | chromosome 10 |
| Location: | 2669663 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437800 | (1 of 3) IPR000104//IPR001054//IPR006059//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Solute-binding family 1 // Nucleotide cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAATTCCCGATGCGCGAGCGGACGTTGGG |
| Internal bar code: | TGGATTCGCGAGCGGCGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 339 |
| LEAP-Seq percent confirming: | 99.5522 |
| LEAP-Seq n confirming: | 667 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGATGACTGCTGCGGCT |
| Suggested primer 2: | CTGACGTACCTTGCTCCCTC |