| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231530 |
| Chromosome: | chromosome 16 |
| Location: | 2304028 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659200 | CYP36,CYP742A1 | (1 of 1) 1.14.13.157 - 1,8-cineole 2-exo-monooxygenase / CYP3A4; Cytochrome P450, CYP4 superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGACTCAGTGGGGCTGCAATGCACAGG |
| Internal bar code: | TTTTGGGTAGGATTATCCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 289 |
| LEAP-Seq percent confirming: | 99.6727 |
| LEAP-Seq n confirming: | 6699 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCGTACAATGAGCTTTT |
| Suggested primer 2: | CTGGTTGAGGTGGTTGGAGT |