Insertion junction: LMJ.RY0402.231530_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231530
Chromosome:chromosome 16
Location:2304448
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g659200 CYP36,CYP742A1 (1 of 1) 1.14.13.157 - 1,8-cineole 2-exo-monooxygenase / CYP3A4; Cytochrome P450, CYP4 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCCACTTCAACCACCTCGAGTACTCCAA
Internal bar code:GAGCGCTGTGGTGAGGCATTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:95.0495
LEAP-Seq n confirming:288
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACAACACGCTGCTGTCTACG
Suggested primer 2:CTCCACAAACCGTGGCTAAT