| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231536 |
| Chromosome: | chromosome 7 |
| Location: | 1233795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g321350 | (1 of 2) 3.1.3.1 - Alkaline phosphatase / Phosphomonoesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATGGAACCCACCCCACCCCTGACAGC |
| Internal bar code: | TTGGAGGGAGGTAACTGAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 697 |
| LEAP-Seq percent confirming: | 98.9937 |
| LEAP-Seq n confirming: | 787 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGCGACCAGAACTACC |
| Suggested primer 2: | AACGAAATCACGCTACCCAC |