| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231543 |
| Chromosome: | chromosome 7 |
| Location: | 5419559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g350350 | FAP217 | Coiled-Coil Flagellar Associated Protein 217 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCGGCTTCCCGGGTCCAGCAGTGGCAT |
| Internal bar code: | GTGCGTAGGAGTGGCCACTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.5017 |
| LEAP-Seq n confirming: | 599 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCTCGGAAGACCAGAGC |
| Suggested primer 2: | CCGTGTGTAGCTGTTGTGCT |