| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231573 |
| Chromosome: | chromosome 12 |
| Location: | 3195881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499100 | (1 of 2) PF01465 - GRIP domain (GRIP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGTGGTTCCGGTGCTGCACCTGCCGC |
| Internal bar code: | GCGCTGATGGGGTAACTTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 99.3694 |
| LEAP-Seq n confirming: | 4570 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCTACCAACGCATGACC |
| Suggested primer 2: | CTACGCTGGTTTGAGAAGGC |