| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231579 |
| Chromosome: | chromosome 9 |
| Location: | 3404482 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388949 | (1 of 1) PTHR10237 - DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLOG SUPPRESSIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACAGCGACGCATTGCAGTTACTCGGTCA |
| Internal bar code: | ATCAATCGGCCTTCGCTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 99.6992 |
| LEAP-Seq n confirming: | 663 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACAATGCAGCGCTAAAG |
| Suggested primer 2: | AACCTGGCCATGCAAGTAAC |