| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231600 |
| Chromosome: | chromosome 3 |
| Location: | 8712630 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204465 | GT90F36,GT90-36 | GT90 family protein 36; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGATAAGCGGCACAGGGCTTTTAAATGT |
| Internal bar code: | GTCCTGGCCGCAACTCTCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 93.865 |
| LEAP-Seq n confirming: | 459 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGGCTGTTGAGGTACA |
| Suggested primer 2: | TGCCCAGAAACCACAATACA |