Insertion junction: LMJ.RY0402.231604_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231604
Chromosome:chromosome 12
Location:5494824
Confidence (%):95
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCATGCCTGGGTGATGCGCACCGAGCTA
Internal bar code:AGGCTTTGCCCTTGTGCCCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:307
LEAP-Seq percent confirming:99.6297
LEAP-Seq n confirming:4036
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCACGTCGCTAGCAATCATA
Suggested primer 2:TGACGCCCTTTTCTCTCTGT