| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231665 |
| Chromosome: | chromosome 7 |
| Location: | 4758688 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g345400 | FAP70 | Flagellar Associated Protein 70; (1 of 1) PTHR23083//PTHR23083:SF418 - TETRATRICOPEPTIDE REPEAT PROTEIN, TPR // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGAGCGGAGCGCCTCACCAAAACTTCCGC |
| Internal bar code: | ACGAAGAGTTTGTGGTTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 318 |
| LEAP-Seq percent confirming: | 88.4615 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACTCCCTCCTTACACAA |
| Suggested primer 2: | GCCGTACTCGTACCACACCT |