Insertion junction: LMJ.RY0402.231678_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.231678
Chromosome:chromosome 2
Location:7310542
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g145350 (1 of 30) PF03407 - Nucleotide-diphospho-sugar transferase (Nucleotid_trans) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCCCCACACACACACAGGGTTCTGTA
Internal bar code:CTCTTGGGAAGGCACCTACGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:760
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1526
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGATGATGAGACTGTGGG
Suggested primer 2:AAACACAGCAGCGACACATC