| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231696 |
| Chromosome: | chromosome 2 |
| Location: | 3500508 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095125 | (1 of 1) IPR000104//IPR000408//IPR009091 - Antifreeze protein, type I // Regulator of chromosome condensation, RCC1 // Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTCAAACACAGGATGCAGTCCCACGCT |
| Internal bar code: | GAACGTGTTCGAAACTTTGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 99.7052 |
| LEAP-Seq n confirming: | 9809 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGCAGGGCAGTATGGG |
| Suggested primer 2: | CGACGTGTGTGTGTGTGTGT |