| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231702 |
| Chromosome: | chromosome 16 |
| Location: | 4239414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g668400 | (1 of 1) PTHR23354//PTHR23354:SF72 - NUCLEOLAR PROTEIN 7/ESTROGEN RECEPTOR COACTIVATOR-RELATED // PROTEIN LMD-3, ISOFORM A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGGCAGGTGTGTACGGGGCATGCGC |
| Internal bar code: | GGCTCGCGTAATGTAGCTGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 99.6238 |
| LEAP-Seq n confirming: | 4767 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCAGCCCAAACTAAACCG |
| Suggested primer 2: | CACCGAATAGACCGCAAAAT |