| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231726 |
| Chromosome: | chromosome_1 |
| Location: | 4310695 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029150 | DAU1,ODA7 | Outer row dynein assembly protein, predominantly cytoplasmic | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTCCTCCTCCTCCTTGAGCTGGTTGCGCAT |
| Internal bar code: | GATAATGTGCGGTCGTCGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 99.5444 |
| LEAP-Seq n confirming: | 437 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGACTGCAAGTAAATGGC |
| Suggested primer 2: | CCTGAAGTGCCTGTGGGTAT |