| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231728 |
| Chromosome: | chromosome 3 |
| Location: | 2596483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160600 | (1 of 1) IPR000104//IPR000679//IPR013088 - Antifreeze protein, type I // Zinc finger, GATA-type // Zinc finger, NHR/GATA-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTCTTGGGTAGCTCTCGCTGTTTAGCTT |
| Internal bar code: | CGTGACTCGGTCAGTTGACCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 98.0186 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCTGTCATAGGTGCTGTC |
| Suggested primer 2: | ACAATCGACACCCTCTGACC |