| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231750 |
| Chromosome: | chromosome 16 |
| Location: | 5366094 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g681700 | NUOS4B | (1 of 2) K03937 - NADH dehydrogenase (ubiquinone) Fe-S protein 4 (NDUFS4); NADH:ubiquinone oxidoreductase subunit | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCCTGTTCTGAACCCTGCGCCTCCAC |
| Internal bar code: | CCGGCTCCACCGGTGTCGACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 920 |
| LEAP-Seq percent confirming: | 98.9305 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGTGCAGCTCGGGTTGT |
| Suggested primer 2: | CACACACGCAGCTAACCACT |