| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231775 |
| Chromosome: | chromosome 12 |
| Location: | 3564047 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513450 | TUH1,TUH | Zeta tubulin; (1 of 1) PTHR11588//PTHR11588:SF6 - TUBULIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACACGGTAATGCTCCAATCCTCGCAC |
| Internal bar code: | CACTAGTCGCTGCGGCTAAGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 355 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGTTTGCCACGAGGAC |
| Suggested primer 2: | TCGAAAGGCTACCAGTGCTT |