| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231777 |
| Chromosome: | chromosome 17 |
| Location: | 3395206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724000 | (1 of 2) PF14908 - Domain of unknown function (DUF4496) (DUF4496) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCTTGACATTGACTTTCAGCTCATGCG |
| Internal bar code: | TTTAGGTGCCGCGCGGTTGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 94.4282 |
| LEAP-Seq n confirming: | 322 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAAGGCTTACTTGAGGG |
| Suggested primer 2: | GGCTGTGTGTCTGTAGGCAA |