| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231802 |
| Chromosome: | chromosome 7 |
| Location: | 3933187 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339250 | (1 of 1) K12611 - mRNA-decapping enzyme 1B (DCP1B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGACAGATTGGCTGTCCTCTGCAAAGG |
| Internal bar code: | CACGTAAAGTTGGATCAGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.6513 |
| LEAP-Seq n confirming: | 2286 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCGGGAACTCCTTGTAG |
| Suggested primer 2: | GGCGTATCAGACGAGGAGAA |