Insertion junction: LMJ.RY0402.231810_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.231810
Chromosome:chromosome 13
Location:3731500
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g589350 FBB6 Flagellar/basal body protein 6; (1 of 1) IPR000679//IPR002035//IPR013694 - Zinc finger, GATA-type // von Willebrand factor, type A // VIT domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCGAAGCGACACGCCCACGGCGCTGGTG
Internal bar code:CATTCAAGTGAGAGCCCAAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:99.6094
LEAP-Seq n confirming:255
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTATACGTGGTGGAGGAC
Suggested primer 2:TCTCTCACACCTTGGGAAGC