| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231812 |
| Chromosome: | chromosome 1 |
| Location: | 2550281 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g014751 | (1 of 7) PF14555 - UBA-like domain (UBA_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCAACCCTATCTAGCCGCCCCTGCTGC |
| Internal bar code: | GGTCCGCGGGGAAGGCTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.4106 |
| LEAP-Seq n confirming: | 506 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCACATGTTCAGGGTCACG |
| Suggested primer 2: | GCTTAAGTTCCAGCGAGGTG |