| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231860 |
| Chromosome: | chromosome 14 |
| Location: | 596414 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611950 | PPR-superfamily protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTATGAAACATGGGACTGCCGACGTCCA |
| Internal bar code: | CGGTGCGCGAGTATCAGTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 436 |
| LEAP-Seq percent confirming: | 99.3712 |
| LEAP-Seq n confirming: | 4899 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTTGGGTACAGGGAAGC |
| Suggested primer 2: | AAGCTACCGTGGTTGGACAC |