| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.231882 |
| Chromosome: | chromosome 2 |
| Location: | 4468530 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g100200 | NUOP3 | NADH:ubiquinone oxidoreductase 22 kDa subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTCGGGCCGCATCATGGCGCTACGCGC |
| Internal bar code: | GGTTCATAGATAGCGACGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 234 |
| LEAP-Seq percent confirming: | 99.4845 |
| LEAP-Seq n confirming: | 193 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAATCACAATCTCGCCCT |
| Suggested primer 2: | CCAACAGTGCCGTAAACCTT |