Insertion junction: LMJ.RY0402.231898_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.231898
Chromosome:chromosome 6
Location:3623022
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278126 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGTTCAGGAACACCTGTGACGCGATAACG
Internal bar code:ACCGGCGCCTTACTCTGATTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:841
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTCTCAACGTCACCACC
Suggested primer 2:AGTCGGTAGTAGGGGTGGCT