| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231916 |
| Chromosome: | chromosome 6 |
| Location: | 1789255 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g262400 | (1 of 1) K14676 - lysophospholipid hydrolase (NTE, NRE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCTCCTTTCCATCGTACACACGCGCGC |
| Internal bar code: | TGGATTCTTACACCCTAGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 586 |
| LEAP-Seq percent confirming: | 99.8634 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTGGTAAACCATTCTGT |
| Suggested primer 2: | TTGTGCTTTCTCTCCTGCCT |