| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.231937 |
| Chromosome: | chromosome_10 |
| Location: | 2068329 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g433050 | DRP8,DRP5B,ARCS | Dynamin-related GTPase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGTCAAGGTGCTGCAACACAGTGCACCCC |
| Internal bar code: | GTAAGACTGCGAACAAGCCATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 84.6154 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGCAGGGACTATTGCTC |
| Suggested primer 2: | TTTTGCAGACTTTGCTGGTG |