| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231937 |
| Chromosome: | chromosome_12 |
| Location: | 4413053 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g520950 | BOP5,DIC4,IC138 | Flagellar inner dynein arm I1 intermediate chain IC138 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCTGCGCGGGATAGCGTACGGCACGGCA |
| Internal bar code: | CCTCCCGAAGCCACTCTTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 701 |
| LEAP-Seq percent confirming: | 98.5755 |
| LEAP-Seq n confirming: | 5536 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACGACACACAAGTCCAG |
| Suggested primer 2: | CCACCTCACACTCAGCTCAA |