| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.231942 |
| Chromosome: | chromosome 12 |
| Location: | 1374318 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g488351 | (1 of 1) IPR001368//IPR009030//IPR011641 - TNFR/NGFR cysteine-rich region // Insulin-like growth factor binding protein, N-terminal // Tyrosine-protein kinase ephrin type A/B receptor-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGTGTTACTGACCGTGCCTGCATATGCG |
| Internal bar code: | ACTAGCAACGCTTTAGCGCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 440 |
| LEAP-Seq percent confirming: | 99.3601 |
| LEAP-Seq n confirming: | 3882 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCTCAAGATGTGCTTTG |
| Suggested primer 2: | GAAGAAGCAGGGCACATGAT |