| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.231975 |
| Chromosome: | chromosome 6 |
| Location: | 3249086 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276450 | (1 of 4) 3.1.2.6 - Hydroxyacylglutathione hydrolase / Glyoxalase II | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAGCACTGGGTGCCTGCCAGAGGTGA |
| Internal bar code: | GACAGGAAGCTGGACGTCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.3533 |
| LEAP-Seq n confirming: | 1229 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGACTCGGATAATTGGGAT |
| Suggested primer 2: | GCACTCGTACCCCTTCACAT |