| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232020 |
| Chromosome: | chromosome 11 |
| Location: | 1196559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467691 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGTTGAATGGGTAGCGATACTTGTCTG |
| Internal bar code: | ATACACGTCGACAAAGCTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 599 |
| LEAP-Seq percent confirming: | 94.704 |
| LEAP-Seq n confirming: | 608 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGCTACGCTTTCAGTTC |
| Suggested primer 2: | TGCCAGAACCTGTGTGGATA |