Insertion junction: LMJ.RY0402.232023_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232023
Chromosome:chromosome 11
Location:876396
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre11.g467653 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGAGCATCACCGGCGCCGTACCATACAC
Internal bar code:TCCGAGCGGAAACGGTCCTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:20
LEAP-Seq percent confirming:99.0969
LEAP-Seq n confirming:1207
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTCATCTGTTCGTTTCG
Suggested primer 2:AGCCCAAAACACTAGGCAGA