| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232039 |
| Chromosome: | chromosome 17 |
| Location: | 1533383 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707300 | PGA3 | Phospholipid/glycerol acyltransferase; (1 of 1) PTHR23063:SF1 - 1-ACYLGLYCEROPHOSPHOCHOLINE O-ACYLTRANSFERASE 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCACACTCACTCTCTTCGCTTGCGGAC |
| Internal bar code: | GGAGTTCTAAGTTACGTGCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 757 |
| LEAP-Seq percent confirming: | 99.4997 |
| LEAP-Seq n confirming: | 3381 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGGTCAGGTGATGTTG |
| Suggested primer 2: | GCAACCAGCGAATACCATTT |