| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.232040 |
| Chromosome: | chromosome 10 |
| Location: | 4347380 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTCGGGATCAGAGGCAAACCCTCATCA |
| Internal bar code: | TAGAACTAGCGTTCGGGCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 98.7342 |
| LEAP-Seq n confirming: | 858 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGACCCAACCACATGAGC |
| Suggested primer 2: | AGATGTCCAAGGTGAGGTGG |