| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232044 |
| Chromosome: | chromosome 6 |
| Location: | 5942205 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288700 | GYD1 | Glycolate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTATTGACCCAGCAGCAGTCGCTGGTATGC |
| Internal bar code: | GGCACTAGCCAAGGATGCCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 741 |
| LEAP-Seq percent confirming: | 99.1279 |
| LEAP-Seq n confirming: | 10230 |
| LEAP-Seq n nonconfirming: | 90 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTTGGTCTCTGTAGGG |
| Suggested primer 2: | AACACCAAGTGAAGGTTGCC |