| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232050 |
| Chromosome: | chromosome 17 |
| Location: | 1666351 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708400 | SUB12 | (1 of 1) 3.4.21.66 - Thermitase / Thermophilic Streptomyces serine protease; Subtilisin-like protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCACCCCACTGGCCCGTGGGGCTGCC |
| Internal bar code: | TTCGACGGTACATCTAGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 132 |
| LEAP-Seq percent confirming: | 95.1807 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTGGCCATGAATAAGCC |
| Suggested primer 2: | TCTGAGACGTGTCGCATTTC |