Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.232058 |
Chromosome: | chromosome_6 |
Location: | 4247866 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278245 | Pheophorbide a oxygenase, Rieske iron-sulfur cluster protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTGTATGCGGTAGTGGTTTTGGGGCTGAGG |
Internal bar code: | CTGCGGGAGCCCCGGTGTATGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 116 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 87 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGAAAGCACCAAGGAGTAG |
Suggested primer 2: | ACACACCCTCTCCACTCCAC |