| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232058 |
| Chromosome: | chromosome 12 |
| Location: | 6132002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536000 | ALA2 | (1 of 3) K14802 - phospholipid-transporting ATPase [EC:3.6.3.1] (DRS2, ATP8A); ATPase, aminophospholipid transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGATCGAGGAGACCATGACCTCCAAGTC |
| Internal bar code: | TTAATCGTAGCGGGCGCCTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1499 |
| LEAP-Seq percent confirming: | 99.5255 |
| LEAP-Seq n confirming: | 10278 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAATGAGATGAGCGGACC |
| Suggested primer 2: | ACATGGAGAACTTTGGTGCC |