Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.232058 |
Chromosome: | chromosome_12 |
Location: | 6132007 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g536000 | ALA2 | ATPase, aminophospholipid transporter | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TATGCATGGAGTTTTACAATGTGCCTGACG |
Internal bar code: | GGCAAAATAGGGCGGTGGTAAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 657 |
LEAP-Seq percent confirming: | 97.2174 |
LEAP-Seq n confirming: | 1118 |
LEAP-Seq n nonconfirming: | 32 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGAATGAGATGAGCGGACC |
Suggested primer 2: | ACATGGAGAACTTTGGTGCC |