Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.232084 |
Chromosome: | chromosome_10 |
Location: | 5448652 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g458600 | FAP245 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGAGAAATGAAGGGGACAGCAGGGCGTG |
Internal bar code: | GATACGCCTTCGGGCTCTCTCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 454 |
LEAP-Seq percent confirming: | 99.5472 |
LEAP-Seq n confirming: | 8575 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACATGCCACACAGTCCAAAA |
Suggested primer 2: | GAAGCCAACCAGAAGCTGAC |