| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.232089 |
| Chromosome: | chromosome 9 |
| Location: | 2797262 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389001 | (1 of 77) IPR001623 - DnaJ domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCCTGCGTGGCAGATGTGCGGCACAG |
| Internal bar code: | TGGCGGTTACTGACTACGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 98.6968 |
| LEAP-Seq n confirming: | 9694 |
| LEAP-Seq n nonconfirming: | 128 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAAGCAGGTGCGTGTTA |
| Suggested primer 2: | TACACGACTCAGTCCTCCCC |