Insertion junction: LMJ.RY0402.232096_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.232096
Chromosome:chromosome 7
Location:3832247
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g338550 (1 of 1) IPR002048//IPR006585//IPR006652//IPR008979//IPR011043//IPR011050 - EF-hand domain // Fucolectin tachylectin-4 pentraxin-1 // Kelch repeat type 1 // Galactose-binding domain-like // Galactose oxidase/kelch, beta-propeller // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCAGCACGCCGCCACCCAGCGCCGCATT
Internal bar code:CTCGCTTGATCGGTCCGACTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:432
LEAP-Seq percent confirming:82.7586
LEAP-Seq n confirming:312
LEAP-Seq n nonconfirming:65
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GAGACGTAGCTCCTGTTGCC
Suggested primer 2:GAGCTGTACGAAGTCCAGGG