| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232101 |
| Chromosome: | chromosome 16 |
| Location: | 5058084 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g684350 | HAR1 | (1 of 2) IPR006115//IPR008927//IPR029154 - 6-phosphogluconate dehydrogenase, NADP-binding // 6-phosphogluconate dehydrogenase C-terminal domain-like // 3-hydroxyisobutyrate dehydrogenase, NAD-binding domain; Possible 3-hydroxyacid dehydrogenase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGGCTGCGGCTGCGGGTCTGGACACG |
| Internal bar code: | GGGGACCGAGTGGGGAGATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 385 |
| LEAP-Seq percent confirming: | 99.7964 |
| LEAP-Seq n confirming: | 3431 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTTTCGTATGAGGTGGT |
| Suggested primer 2: | CCAGGAGTCCAACATCCACT |