Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.232105 |
Chromosome: | chromosome 16 |
Location: | 4990569 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g685050 | LCI15 | PRLI-interacting factor L | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGTCGATCGTTGGGCGTACGCTGCGTTT |
Internal bar code: | GGTGGGGATACTGAGGTTCGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 547 |
LEAP-Seq percent confirming: | 98.8204 |
LEAP-Seq n confirming: | 754 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCCACCAGGTCACAGTACCA |
Suggested primer 2: | ACTACCACGGCGAACAAATC |