Insertion junction: LMJ.RY0402.232105_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.232105
Chromosome:chromosome 16
Location:4990569
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g685050 LCI15 PRLI-interacting factor L 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTCGATCGTTGGGCGTACGCTGCGTTT
Internal bar code:GGTGGGGATACTGAGGTTCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:98.8204
LEAP-Seq n confirming:754
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TCCACCAGGTCACAGTACCA
Suggested primer 2:ACTACCACGGCGAACAAATC