| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.232105 |
| Chromosome: | chromosome 16 |
| Location: | 4990581 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685050 | LCI15 | PRLI-interacting factor L | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTTAACACGGCGTGTTTACCCCTGCAA |
| Internal bar code: | ACTTCTGGCCTCAGCCTGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.5562 |
| LEAP-Seq n confirming: | 673 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACCACGGCGAACAAATC |
| Suggested primer 2: | TCCACCAGGTCACAGTACCA |