| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.232106 |
| Chromosome: | chromosome 3 |
| Location: | 3880227 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGCCCCCCAGGGTGAAGGTGGACAGCAC |
| Internal bar code: | CCGGGAGAAATTATCGTTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 154 |
| LEAP-Seq percent confirming: | 98.0558 |
| LEAP-Seq n confirming: | 1160 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGTTATTGTGGGCTGTT |
| Suggested primer 2: | ACTGCACCAGTTGCTGACAC |